Short Course Programme Chromatographic Methods of Protein Purification (CPD)

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Short Course Programme Chromatographic Methods of Protein Purification (CPD)

  • Objectives Aimed at individuals originating from the biomedical sciences, but lacking a bioanalytical background, this course aims to consider both the theoretical principles and practical aspects of the chromatographic separation of proteins and peptides.
  • Entry requirements Entry requirements

    Candidates will normally be expected to possess one of the following qualifications before entry:



    * An Honours degree (at least a 2.2, or its equivalent) in a relevant biological subject from a UK (or equivalent) University

    * A professional qualification of equivalent status

    * Any other such qualification and experience deemed equivalent in subject content and level of attainment by the Admissions Panel



    In addition a good command of English is essential to enable successful completion of the programmes.



    * The minimum requirement is the British Council IELTS grade 6.5 or its equivalent
  • Academic title Short Course Programme Chromatographic Methods of Protein Purification (CPD)
  • Course description What do you study?

        * Principles of chromatography. Chromatographic performance parameters (capacity, plate height and resolution)
        * Selection of the appropriate phases for the separation of peptides and proteins
        * Sample and solvent/buffer preparation (solid phase extraction etc.)
        * Protein purification strategy
        * The use of reversed phase, ion exchange, size exclusion, hydrophobic interaction, affinity and mixed mode chromatography to separate proteins and peptides
        * The use of immobilised metal ion affinity chromatography to separate post-translationally modified proteins and peptides
        * An introduction to the apparatus used including HPLC and protein purification apparatus
        * Data analysis

    Assessment
    Assessment will comprise completion of a written assignment (60%) and a computer-based test on peptides, proteins and separation techniques (40%)

    Why study at Nottingham Trent University?
    Expert tuition by researchers, consultants and professionals.

    Four month access to NTU student resources.

    Your future career

        * This course offers a great opportunity for personal development and career progression.
        * Masters level training in selected advanced topics within the molecular biosciences
        * The learning skills required to apply the techniques acquired to your workplace and/or future careers
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